In a cell free system , binding of a DNA fragment ( 338 303 ) containing a __NODE__ response element from __SP__ __NODE__ gene and __SP__ __NODE__ protein is greater than binding of a DNA fragment ( 338 303 ) containing a __NODE__ response element from mutant __SP__ __NODE__ gene ( T 330A G 329A G 327C G 326T with its __NODE__ response element 1 mutated ) and __SP__ __NODE__ protein .