In a cell free system , binding of promoter fragment ( 67 32 ) from __SP__ __NODE__ gene and mutant __NODE__ protein ( C terminal truncation 461 End ) is greater than binding of promoter fragment ( 67 31 ) from mutant __SP__ __NODE__ gene ( G 61T T 60A C 59T A 58C C 57T C 56A C 52T G 49T ) and mutant __NODE__ protein ( C terminal truncation 461 End ) .