In a cell free system , binding of __SP__ __NODE__ protein and a protein fragment ( 100 411 ) containing a LXXLL motif from __SP__ __NODE__ protein is greater than binding of mutant __SP__ __NODE__ protein ( C terminal truncation 472 End with its AF 2 domain deleted ) and a protein fragment ( 100 411 ) containing a LXXLL motif from __SP__ __NODE__ protein .