In a cell free system , binding of promoter fragment ( 7836 7491 ) from __SP__ __NODE__ gene and a protein protein complex consisting of __SP__ __NODE__ and of __SP__ __NODE__ is greater than binding of mutant promoter fragment ( 7836 7491 ) ( with its __NODE__ 8 element mutated ) from __SP__ __NODE__ gene and a protein protein complex consisting of __SP__ __NODE__ and of __SP__ __NODE__ .