In a cell free system , binding of promoter fragment ( 97 78 ) from __SP__ __NODE__ gene consisting of PPRE and __SP__ __NODE__ protein is greater than binding of mutant promoter fragment ( 97 78 ) ( T 97A G 96A A 95G C 93T with its PPRE mutated ) from __SP__ __NODE__ gene consisting of PPRE and __SP__ __NODE__ protein .