In a cell free system , binding of promoter fragment ( 67 32 ) from __SP__ __NODE__ gene and mutant __NODE__ protein ( C terminal truncation 461 End ) is greater than binding of promoter fragment ( 67 34 ) from mutant __SP__ __NODE__ gene ( G 61T T 60C C 59A A 58C C 57A C 56A T 55C G 54T A 53C C 52A ) and mutant __NODE__ protein ( C terminal truncation 461 End ) .