In a cell free system , binding of promoter fragment ( 766 734 ) from __SP__ __NODE__ gene and mutant __NODE__ protein ( C terminal truncation 461 End ) is greater than binding of promoter fragment ( 766 734 ) from mutant __SP__ __NODE__ gene ( T 752G C 751A A 750C T 747G G 746A C 743A A 742C T 739G G 738T ) and mutant __NODE__ protein ( C terminal truncation 461 End ) .