In a cell free system , the affinity of binding of promoter fragment ( 68 51 ) containing a __NODE__ response element 1 from __NODE__ gene and mutant __NODE__ protein ( C terminal truncation 461 End ) is greater than the affinity of binding of promoter fragment ( 68 51 ) containing a __NODE__ response element 1 from __NODE__ gene and mutant __NODE__ protein ( K359I C terminal truncation 461 End ) .