In a cell free system , the affinity of binding of promoter fragment ( 68 51 ) containing a __NODE__ response element 1 from __NODE__ gene and mutant __NODE__ protein ( C terminal truncation 461 End ) is the same as the affinity of binding of promoter fragment ( 67 32 ) from __SP__ __NODE__ gene and mutant __NODE__ protein ( C terminal truncation 461 End ) .