In a system of purified components , binding of a DNA fragment ( GATCTGTGAACTCTGATCCAGTAAG ) from __NODE__ response element and __SP__ __NODE__ protein and __SP__ __NODE__ protein is the same as binding of a DNA fragment ( GATCTGTGAACTCTGATCCAGTAAG ) from __NODE__ response element and dominant negative mutant __SP__ __NODE__ protein ( C terminal truncation ) and __SP__ __NODE__ protein .