In a system of purified components , binding of a immobilized protein fragment containing a extracellular domain from __NODE__ protein and a soluble protein fragment containing a extracellular domain from __NODE__ protein is greater than binding of a immobilized mutant protein fragment ( F117S with its __NODE__ homology domain 3 mutated ) containing a extracellular domain from __NODE__ protein and a soluble protein fragment containing a extracellular domain from __NODE__ protein .