In a system of purified components , binding of EcRE ( 5 ' AGTGCATTGACCT 3 ' from mutant __NODE__ gene(s) ( A?C C?T ) DNA endogenous promoter and a protein protein complex consisting of __NODE__ and of __SP__ __NODE__ is greater than binding of EcRE ( 5 ' AGTGCATTGAACC 3 ' from __NODE__ gene(s) DNA endogenous promoter and a protein protein complex consisting of __NODE__ and of __SP__ __NODE__ .