In cell free system , binding of a DNA fragment ( 225 150 ) containing a multiple copies ( 2 times ) __NODE__ regulatory element from __SP__ __NODE__ gene and __SP__ __NODE__ protein is greater than binding of a mutant DNA fragment ( 225 150 ) ( C 178T A 177T with its __NODE__ regulatory element 2 mutated ) containing a multiple copies ( 2 times ) __NODE__ regulatory element from __SP__ __NODE__ gene and __SP__ __NODE__ protein .