In cell free system , binding of a DNA fragment ( 225 150 ) containing a multiple copies ( 2 times ) __NODE__ regulatory element from __SP__ __NODE__ gene and __SP__ __NODE__ protein is greater than binding of a mutant DNA fragment ( 225 150 ) ( T 199A G 198A with its __NODE__ regulatory element 1 mutated ) containing a multiple copies ( 2 times ) __NODE__ regulatory element from __SP__ __NODE__ gene and __SP__ __NODE__ protein .