In a cell free system , binding of a protein chimera composed of mutant __NODE__ 6 [__NODE__] protein ( N terminal truncation with its N terminal domain deleted and with its C terminal domain and its central domain retained ) and of N terminal domain from __NODE__ 5 [__NODE__] protein and __SP__ __NODE__ protein is greater than binding of a protein chimera composed of mutant __NODE__ 6 [__NODE__] protein ( N terminal truncation with its N terminal domain deleted and with its C terminal domain and its central domain retained ) and of N terminal domain from __NODE__ 5 [__NODE__] protein and __SP__ __NODE__ protein .