In a cell free system , binding of mutant __NODE__ protein ( N terminal truncation 1 243 with its __NODE__ finger domain 1 deleted and with its __NODE__ finger domain 2 retained ) and __NODE__ protein is greater than binding of mutant __NODE__ protein ( C290S C293S with its __NODE__ finger domain 2 mutated N terminal truncation 1 243 with its __NODE__ finger domain 1 deleted and with its __NODE__ finger domain 2 retained ) and __NODE__ protein .