In a cell free system , binding of a DNA fragment ( TCGACTTCCAAAATGGGGATTAAATGAGGTAATATC ) from __SP__ __NODE__ gene and a protein protein complex consisting of __NODE__ and of __NODE__ is greater than binding of a DNA fragment ( TCGACTTCCAAAATGGTTATTAAATGAGGTAATATC ) from mutant __SP__ __NODE__ gene ( G?T G?T ) and a protein protein complex consisting of __NODE__ and of __NODE__ .