In a cell free system , binding of mutant __NODE__ protein ( C terminal truncation 404 End with its AF 2 transcription activation domain deleted ) and mutant __NODE__ protein ( C terminal truncation 339 End ) is the same as binding of mutant __NODE__ protein ( C terminal truncation 360 End with its AF 2 transcription activation domain deleted and with its ligand binding domain mutated ) and mutant __NODE__ protein ( C terminal truncation 339 End ) .