In a cell free system , binding of a protein fragment ( 1762 1960 ) containing a pdz domain 4 5 from __NODE__ protein and a protein fragment ( aa sequence ASLV ) from mutant __NODE__ protein ( Q?A ) is greater than binding of a protein fragment ( 1762 1960 ) containing a pdz domain 4 5 from __NODE__ protein and a protein fragment ( aa sequence QSLV ) from __NODE__ protein .