In a cell free system , binding of a protein fragment ( 1086 1449 ) from __SP__ __NODE__ protein and a heterodimeric protein protein complex consisting of __NODE__ and of __SP__ __NODE__ and __NODE__ response element from __SP__ Lysozyme gene is greater than binding of a protein fragment ( 1086 1449 ) from __SP__ __NODE__ protein and a heterodimeric protein protein complex consisting of __NODE__ and of mutant __SP__ __NODE__ ( M259I ) and __NODE__ response element from __SP__ Lysozyme gene .